Herskowitz Lab Protocol
From : RKT 7/96

pNKY51 Sequence (Assemble based on published information):

gaattcagatcttccagtggtgcatgaacgcatgagaaagcccccggaagatcatcttccgggggctttttttttggcgc

gcgatacagaccggttcagacaggataaagaggaacgcagaatgttagacaacacccgcttacgcatagctattcagaaa

tcaggccgtttaagcgatgattcacgagaattgctggcccgctgcggcataaaaattaatttacacactcagcgcctgat

tgcgatggcggaaaacatgccgattgatatcctgcgcgtgcgtgatgatgacattccgggtctggtaatggatggcgtgg

tcgatctcggtattatcggcgaaaacgtgctggaagaagagctactcaaccgccgcgcacagggcgaagatccacgctat

ttaaccctgcgccgtcttgacttcggcggctgccgtttatcgctggcaacaccggttgacgaagcctgggacggcccggc

cgcgctggacggtaaacgtatcgctacctcatatccgcacctcctcaaacgctacctcgaccagaaaggcgtctctttta

aatcgtgtctgttaaatggttctgtcgaagtcgcgccgcgcgcggggctggccgacgctatctgcgatttggtctctacc

ggcgcgacgcttgaagctaacggcctgcgtgaagtcgaagttatctaccgctctaaagcctgtctgattcagcgcgacgg

tgagatggcacagagcaagcaagagctgatcgataaattgctgacccgtattcagggcgtgattcaggcgcgcgaatcga

aatacatcatgatgcacgcgccaagtgaacgcctggaagaggttatcgccctgctgccaggcgccgaaaggccgacaatt

ctgccgctggcaggcgagcaacagcgcgtggcgatgcacatggtcagcagcgaaacgttgttctgggaaaccatggagaa

actgaaagcgcttggcgccagctcgattctggtactgccgatcgagaagatgatggagtgatctgacgcctgatggcgct

gcgcttatcaggcctacgtaatgcgttgatattttgggttctgtaggccggataaggcggaaccctgtgatggagtaaag

accatgagcttcaataccctgattgactggaacagggatctgaattaattctcatgtttgacagcttatcatcgataagc

ttttcaattcaattcatcattttttttttattcttttttttgatttcggtttctttgaaatttttttgattcggtaatct

ccgaacagaaggaagaacgaaggaaggagcacagacttagattggtatatatacgcatatgtagtgttgaagaaacatga

aattgcccagtattcttaacccaactgcacagaacaaaaacctgcaggaaacgaagataaatcatgtcgaaagctacata

taaggaacgtgctgctactcatcctagtcctgttgctgccaagctatttaatatcatgcacgaaaagcaaacaaacttgt

gtgcttcattggatgttcgtaccaccaaggaattactggagttagttgaagcattaggtcccaaaatttgtttactaaaa

acacatgtggatatcttgactgatttttccatggagggcacagttaagccgctaaaggcattatccgccaagtacaattt

tttactcttcgaagacagaaaatttgctgacattggtaatacagtcaaattgcagtactctgcgggtgtatacagaatag

cagaatgggcagacattacgaatgcacacggtgtggtgggcccaggtattgttagcggtttgaagcaggcggcagaagaa

gtaacaaaggaacctagaggccttttgatgttagcagaattgtcatgcaagggctccctatctactggagaatatactaa

gggtactgttgacattgcgaagagcgacaaagattttgttatcggctttattgctcaaagagacatgggtggaagagatg

aaggttacgattggttgattatgacacccggtgtgggtttagatgacaagggagacgcattgggtcaacagtatagaacc

gtggatgatgtggtctctacaggatctgacattattattgttggaagaggactatttgcaaagggaagggatgctaaggt

agagggtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcggccagcaaaactaaaaaactgtattat

aagtaaatgcatgtatactaaactcacaaattagagcttcaatttaattatatcagttattacccgggaatctcggtcgt

aatgatttttataatgacgaaaaaaaaaaaattggaaagaaaaagctttaatgcggtagtttatcacagttaaattgcta

acgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggca

taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtg

ctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgccc

agtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgTggatcttccagt

ggtgcatgaacgcatgagaaagcccccggaagatcatcttccgggggctttttttttggcgcgcgatacagaccggttca

gacaggataaagaggaacgcagaatgttagacaacacccgcttacgcatagctattcagaaatcaggccgtttaagcgat

gattcacgagaattgctggcccgctgcggcataaaaattaatttacacactcagcgcctgattgcgatggcggaaaacat

gccgattgatatcctgcgcgtgcgtgatgatgacattccgggtctggtaatggatggcgtggtcgatctcggtattatcg

gcgaaaacgtgctggaagaagagctactcaaccgccgcgcacagggcgaagatccacgctatttaaccctgcgccgtctt

gacttcggcggctgccgtttatcgctggcaacaccggttgacgaagcctgggacggcccggccgcgctggacggtaaacg

tatcgctacctcatatccgcacctcctcaaacgctacctcgaccagaaaggcgtctcttttaaatcgtgtctgttaaatg

gttctgtcgaagtcgcgccgcgcgcggggctggccgacgctatctgcgatttggtctctaccggcgcgacgcttgaagct

aacggcctgcgtgaagtcgaagttatctaccgctctaaagcctgtctgattcagcgcgacggtgagatggcacagagcaa

gcaagagctgatcgataaattgctgacccgtattcagggcgtgattcaggcgcgcgaatcgaaatacatcatgatgcacg

cgccaagtgaacgcctggaagaggttatcgccctgctgccaggcgccgaaaggccgacaattctgccgctggcaggcgag

caacagcgcgtggcgatgcacatggtcagcagcgaaacgttgttctgggaaaccatggagaaactgaaagcgcttggcgc

cagctcgattctggtactgccgatcgagaagatgatggagtgatctgacgcctgatggcgctgcgcttatcaggcctacg

taatgcgttgatattttgggttctgtaggccggataaggcggaaccctgtgatggagtaaagaccatgagcttcaatacc

ctgattgactggaacagggatccTctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcg

cctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatg

gtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacgg

cctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttca

acccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactc

gtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtc

gcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagc

aggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttc

cccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacga

ccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcga

tttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcg

ttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccactcca

agaattggagccaatcaattcttgcggagaactgtgaatgcgcaaaccaacccttggcagaacatatccatcgcgtccgc

catctccagcagccgcacgcggcgcatctcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgt

tgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgac

tgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctggaaacgcg

gaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacctacatctg

tattaacgaagcgctggcattgaccctgagtgatttttctctggtcccgccgcatccataccgccagttgtttaccctca

caacgttccagtaaccgggcatgttcatcatcagtaacccgtatcgtgagcatcctctctcgtttcatcggtatcattac

ccccatgaacagaaattcccccttacacggaggcatcaagtgaccaaacaggaaaaaaccgcccttaacatggcccgctt

tatcagaagccagacattaacgcttctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtgaatcgc

ttcacgaccacgctgatgagctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcag

ctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttgg

cgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagca

gattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctctt

ccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaata

cggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaa

ggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggc

gaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccg

cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttc

ggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaact

atcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgagg

tatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgc

tctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt

tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgac

gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaa

ttaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgagg

cacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggag

ggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacca

gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaag

ctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcg

tttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggt

tagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcata

attctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgt

atgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcat

cattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtg

cacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggca

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