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The Wee-P Protein Trap:
Tagging Proteins With GFP at Their Native Genomic Loci

I.	Introduction
II.	Methods
III.	Using Wee-Ps in P-element replacement experiments
IV.	Table of available fusion lines
V.	Table of expression patterns and images for Wee-P lines whose gene has not been determined

V. Table of expression patterns and images for available Wee-P lines whose gene has not been determined

Description of Column Headings:

Expression pattern (first instar): this is the expression pattern that was noted when the animal was collected. Thus it is not a dissected animal and is just the expression pattern as seen through the cuticle. It is thus by no means an exhaustive list of tissues in which the GFP might be found.

Expression pattern (third instar): this is the expression pattern as seen in a filleted third instar. Here the list of tissues is more detailed, but note that many tissues were not examined, such as the salivary glands or the many tissues of the intestinal tract.

Due to the highly specific expression patterns of the lines in this table, both at the tissue level and, more importantly at the subcellular level, we believe that the majority will prove to be real fusions as opposed to enhancer trap-like events. As most of these lines are not at the neuromuscular junction, we will most likely not be following up on them.

Line #	Expression pattern (first instar)	Expression pattern (dissected third instar)
WeeP1500	Faint in CNS axons	CNS neuropil, Axons, PNS(?) MAP?
WeeP1502	CNS cell bodies, Muscles	CNS cell bodies, Axons, possibly NMJ
WeeP1503	CNS cell bodies, gonads, elsewhere	CNS, Imaginal Discs cytoplasmic, Axon membranes
WeeP1504	Fat bodies and CNS	Faint in VNC cell bodies, in subset of CNS cell bodies, Salivary glands associated with membranes
WeeP1507	CNS cell bodies	Pattern in CNS cell bodies, imaginal discs
WeeP1508	Bright throughout body, CNS nuclei	Bright in all nuclei
WeeP1509	Bright in CNS
WeeP1511	CNS throughout	CNS cell bodies, imaginal discs
WeeP1512	CNS cell bodies, nuclei of all cells	CNS cell bodies, nuclei of all cells
WeeP1513	CNS dim, elsewhere in animal	Throughout CNS, compartment boundaries of discs
WeeP1514	CNS	CNS cell bodies and connectives, eye discs, faint in imaginal discs
WeeP1515	CNS all	CNS cell bodies, faint in neuropil, imaginal discs
WeeP1517	CNS all, muscles	CNS cell bodies, subset, connectives, axons, muscles
WeeP1519	Fat bodies	CNS cell bodies, faint
WeeP1520	Nuclei of all cells	Nuclei of all cells
WeeP1522	CNS faint	Faint in CNS
WeeP1523	Bright in CNS, anterior and elsewhere
WeeP1524	Bright in CNS
WeeP1525	CNS, faint in center
WeeP1526	Interior (gut?)	Cytoplasmic in all cell types. Cell bodies of CNS, also in midline of VNC
WeeP1527	CNS dim	CNS and VNC cell bodies, faint in imaginal discs
WeeP1528	CNS all	Bright in CNS, VNC cell bodies, faint in imaginal discs
WeeP1529	Bright in CNS	Bright in CNS, VNC, bright in proximal axons, fades as the axon leaves VNC. Enriched in optic anlage, eye discs
WeeP1530	CNS	CNS, faint in axons
WeeP1531	Cuticle	Cuticle, tracheal cells
WeeP1532	Dim in anterior portion of animal
WeeP1533	CNS all
WeeP1534	CNS all
WeeP1535	CNS all
WeeP1536	CNS all
WeeP1537	CNS axons
WeeP1538	CNS bright
WeeP1539	GFP throughout
WeeP1540	Very dim in CNS outer
WeeP1541	CNS outer, dim
WeeP1542	CNS all
WeeP1543	Dim GFP throughout	GFP in muscles
WeeP1544	Dim GFP throughout
WeeP1545	CNS bright
WeeP1546	Diffuse GFP in anterior	Subset of CNS and VNC cell bodies, axons throughout length, also in imaginal discs. Protein was membrane bound and speckled.
WeeP1547	Dim down center
WeeP1548	Fat bodies	GFP in cytosol of all cell types. Found in axons throughout length, however, did not see it at the synapse. As expression level was so high I don't think there is any at NMJ.
WeeP1549	Dim in anterior CNS outer	Nuclei of CNS cell bodies, discs and epithelial. Enrichment at D-V boundary in wing discs
WeeP1550	Dim in anterior
WeeP1551	CNS dim
WeeP1552	Dim in anterior	CNS and imaginal discs, cell bodies
WeeP1553	Bright in interior, CNS all	Brightly expressed in cytosol of CNS cell bodies, in axons faintly, also in epithelial cells and gut (cytosolic)
WeeP1554	CNS all	Cytosol of CNS cell bodies, in neuropil. Patterned in ID. Only in ommatidia of eyes. Not at NMJ
WeeP1555	CNS axons	Cytosol of CNS and eyedisc cell bodies
WeeP1556	Dim throughout
WeeP1557	CNS, dim	Bright expression in CNS cell bodies and dimly in imaginal discs
WeeP1558	Faint in CNS
WeeP1559	CNS bright
WeeP1560	Bright throughout	CNS cell bodies and VNC midline imaginal discs with possible D-V enrichment
WeeP1561	Dim in anterior
WeeP1562 (Ph1)	Dim throughout, enriched in posterior	Synaptic neuropil and CNS axons. Axons faint.
WeeP1563 (Ph1)	Dim in CNS	Cytoplasm of CNS and VNC, nowhere else.
WeeP1564 (Ph1)	Bright in gut, dim in CNS diffuse throughout	Bright in gut, synaptic neuropil, faint in axons.
WeeP1565 (Ph1)	CNS, PNS, gut wall, elsewhere
WeeP1566 (Ph1)	Dim in CNS, diffuse throughout	Faint in muscles? Check 1st instar to be sure.
WeeP1567 (Ph1)	CNS cell bodies	Bright in nuclei of several cell types. CNS, muscles, muscle attachment cells.
WeeP1568 (ph1)	GFP throughout body	Cytosolic in CNS, discs. Also in trachea and epithelials. Faint in axons.
WeeP1569 (Ph1)	CNS all, dim in anterior	Throughout CNS, discs membranes. Axons.
WeeP1570 (Ph1)	Dim in CNS all	Imaginal discs faintly
WeeP1571 (ph1)	GFP throughout	Bright in CNS and imaginal discs. Muscle attachements and muscles.
WeeP1572 (ph1)	CNS
WeeP1573 (Ph1)	CNS

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The Wee-P Protein Trap: Tagging Proteins With GFP at Their Native Genomic Loci

The Wee-P Protein Trap:
Tagging Proteins With GFP at Their Native Genomic Loci