guthrie lab publications

Guthrie Lab Publications — 1985 - present


 
  indexed by date
 
 
    2005 - present

  1. Kress, T.L., Krogan, N.J. and Guthrie, C. (2008) A single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast. Mol. Cell. In press.

  2. Maeder, C. and Guthrie, C. (2008) Modifications target spliceosome dynamics. Nat Struct Mol Biol. 15(5):426-8. [Pubmed citation].

  3. Lund, M.K., Kress, T.L. and Guthrie, C. (2008) Autoregulation of Npl3, a yeast SR protein, requires a novel downstream region and serine phosphorylation. Mol Cell Biol. 28(11):3873-81. [Pubmed citation]

  4. Mitrovich, Q.M. and Guthrie, C. (2007) Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts. RNA 13: 2066-80. [Pubmed citation]

  5. Pleiss, J., Whitworth, G., Bergkessel, M. and Guthrie, C. (2007) Rapid, transcript-specific changes in splicing in response to environmental stress. Mol. Cell  27, 928–937. [Pubmed citation]

  6. Pleiss, J., Whitworth, G., Bergkessel, M. and Guthrie, C. (2007) Defects in essential spliceosomal components differentially affect splicing in a transcript-specific manner. PLoS Biol 5(4): e90. [Pubmed citation]

  7. Mitrovich, Q., Tuch, B.B., Guthrie, C. and Johnson, A.D. (2007) Computational and experimental approaches double the number of known introns in Candida alibcans. Genome Res. 17:492-502. [Pubmed citation]

  8. Kim-Guisbert, K., Li, H. and Guthrie, C. (2006) Alternative 3’ end processing in Saccharomyces cerevisiae is modulated by Nab4/Hrp1. PLoS Biol. 2006 Dec: 5(1):e6 [Pubmed citation]

  9. Kress. T and Guthrie, C. (2006) Accurate RNA “siting” and splicing gets help from a DEK-hand (Perspective) Science 312:1886-1887 [Pubmed citation].

  10. Preker, P. and Guthrie, C. (2006) Autoregulation of the mRNA export factor Yra1p requires inefficient splicing of its pre-mRNA. RNA 12: 994-106. [Pubmed citation]

  11. Brenner, T. and Guthrie, C. (2006). Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain. RNA  12: 862-8 [Pubmed citation]

  12. Bellare, P., Kutach, A.K., Rines, A.K., Guthrie, C. and Sontheimer, E.J. (2005) Ubiquitin binding by a varant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA 12:292-302. [Pubmed citation]

  13. Lund, M. and Guthrie, C. (2005) The DEAD-box ATPase Dbp5 is required to dissociate Mex67p from exported mRNPs at the nuclear rim. Molec. Cell, 20(4):645-651 [Pubmed citation]

  14. Villa, T. and Guthrie, C. (2005) The Isy1p component of the NineTeen Complex interacts with the ATPase Prp16p to regualte the fidelity of pre-mRNA splicing. Genes and Dev., 19:1894-1904. [Pubmed citation]

  15. Guthrie, C. and Steitz, J. (2005) Nucleus and gene expression: Coordinated nuclear events regulate mRNA synthesis, processing, export and turnover. (Editorial Overview) Curr. Opin. Cell Biol., 17:239-241.

  16. Brenner, T. and Guthrie, C. (2005) Genetic analysis reveals a role for the C-terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics, 170:1063-1080. [ Pubmed Citation]

  17. Kim Guisbert, K., Duncan, K., Li, H., and Guthrie, C. (2005) Functional specificity of shuttling hnRNPs revealed by genome-wide analysis of their RNA binding profiles. RNA. [2005 Feb 9, Epub ahead of print]. [ Pubmed Citation] [Supplementary Information]


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    2000 - 2004

  18. Gilbert, W. and Guthrie, C. (2004) The Glc7p nuclear phosphatase promotes mRNA export by facilitating association of Mex67p with mRNA. Mol Cell, 13(2):201-12. [Pubmed Citation]

  19. Inada, M. and Guthrie, C. (2004) Identification of Lhp1p-associated RNAs by microarray analysis in Saccharomyces cerevisiae reveals association with coding and noncoding RNAs. PNAS, 101(2):434-9. Epub 2004 Jan 02. [Pubmed Citation]

  20. Rader, S.D. and Guthrie, C. (2002) A conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation. RNA, 8(11):1378-92 [Pubmed citation]

  21. Preker, P.J., Kim, K.S. and Guthrie, C. (2002) Expression of the essential mRNA export factor Yra1p is autoregulated by a spicing-dependent mechanism. RNA, 8(8):969-80 [Pubmed citation]

  22. Villa, T., Pleiss, J.A. and Guthrie, C. (2002) Spliceosomal snRNAs: Mg(2+)-dependent chemistry at the catalytic core? Cell 109(2):149-52. [Pubmed citation]

  23. Collins, C.A. and Guthrie, C. (2001) Genetic interactions between the 5' and 3' splice site consensus sequences and U6 during the second catalytic step of pre-mRNA splicing. RNA 7(12):1845-54. [Pubmed citation]

  24. de Bruyn Kops, A. and Guthrie, C. (2001) An essential nuclear envelope integral membrane protein, Brr6p, required for nuclear transport. EMBO J 20(15):4183-93. [Pubmed citation]

  25. Gilbert, W., Siebel, C.W. and Guthrie, C. (2001) Phosphorylation by Sky1p promotes Npl3p shuttling and mRNA dissociation., RNA, 7:1-12 [PubMed citation]

  26. Kistler, A.L., and Guthrie, C. (2001) Deletion of MUD2, the yeast homologue of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase. Genes & Develop., 15:42-9. [PubMed citation]

  27. Collins, C. and Guthrie C. (2000) The question remains: Is the spliceosome a ribozyme? Nat. Struc. Biol. 7:850-854. [PubMed citation]


  28. Duncan, K., Umen, James G. and Guthrie, C.. (2000) A putative ubiquitin ligase required for efficient mRNA export differentially affects hnRNP transport. Current Biology 10: 687-696. [PubMed citation]


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    1995 - 1999

  29. Collins, C. and Guthrie, C. (1999) Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome. Genes & Develop. 13:1970-1982 [PubMed citation]


  30. Siebel, C.W., Feng, L., Guthrie, C. and Fu, X.-D. (1999) Conservation in budding yeast of a kinase specific for SR factors. Proc. Natl. Acad. Sci., 96: 5440-5445. [PubMed citation]


  31. Staley, J.P. and Guthrie, C. (1999) An RNA switch at the 5' splice site requires ATP and the DEAD-box protein Prp28p. Molec. Cell, 3: 55-64. [PubMed citation]


  32. Wang, Y. and Guthrie, C. (1998) Prp16, a DEAH-box RNA helicase, is recruited onto the spliceosome primarily via its non-conserved N-terminal domain. RNA, 4: 1216-1229. [PubMed citation]


  33. Ragunathan, P. and Guthrie, C. (1998) RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr. Biol., 8: 847-855. [PubMed citation]


  34. Wang, Y., Wagner, J. and Guthrie, C. (1998) Prp16, a DEAH-box splicing factor, unwinds RNA duplexes in vitro. Curr. Biol., 8: 441-451. [PubMed citation]


  35. Staley, J.P. and Guthrie, C. (1998) Mechanical devices in the spliceosome: Clocks, motors, springs and things. Cell, 92: 315-326. [PubMed citation]


  36. Raghunathan, P.L. and Guthrie, C. (1998) A spliceosomal recycling factor that reanneals U4 and U6 snRNPs. Science, 279: 857-860. [PubMed citation]


  37. Stade, K., Ford, C.S., Guthrie, C. and Weis, K. (1997) Exportin 1 (Crm1p) is an essential nuclear export factor. Cell, 90: 1041-1050. [PubMed citation]


  38. Chanfreau, G., Elela, S.A., Ares, M. and Guthrie, C. (1997) Alternative 3'-end processing of U5 snRNA by RNase III. Genes & Devel., 11: 2741-2751. [PubMed citation]


  39. Siebel, C.W. and Guthrie, C. (1996) The essential yeast RNA binding protein Np13p is methylated. Proc. Natl. Acad. Sci., 93: 13641-13646. [PubMed citation]


  40. Chanfreau, G., Noble, S.M. and Guthrie, C. (1996) An essential yeast protein with unexpected similarity to subunits of mammalian cleavage and polyadenylation specificity factor (CPSF). Science, 274: 1511-1514. [PubMed citation]


  41. Noble, S. and Guthrie, C. (1996) Transcriptional pulse-chase analysis reveals a role for a novel snRNP-associated protein in the manufacture of spliceosomal snRNPs. EMBO J, 15: 4368-4379. [PubMed citation]


  42. Noble, S. and Guthrie, C. (1996) Identification of novel components of the yeast pre-mRNA splicing machinery by means of cold-sensitive mutants. Genetics, 143: 67-80. [PubMed citation]


  43. Umen, J. and Guthrie, C. (1996) Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3' splice site selection. Genetics, 143: 723-739. [PubMed citation]


  44. Guthrie, C. (1996) The spliceosome is a dynamic ribonucleoprotein machine. Harvey Lecture Series, 90: 59-80. [PubMed citation]


  45. Umen, J. and Guthrie, C. (1995) The second catalytic step of pre-mRNA splicing. RNA, 1: 869-885. [PubMed citation]


  46. Umen, J. G. and Guthrie, C. (1995) Prp16, Slu7 and Prp8 interact with the 3' splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA, 1:584-597. [PubMed citation]


  47. Jones, M.H., Frank, D.N., and Guthrie, C. (1995) Characterization and functional ordering of Slu7p and Prp17p during the second step of premRNA splicing in yeast. Proc. Natl. Acad. Sci., 92: 9687-9691. [PubMed citation]


  48. Jandrositz, A., and Guthrie, C. (1995) Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs. EMBO J., 14: 820-832. [PubMed citation]


  49. Umen, J. and Guthrie, C. (1995) A novel role for a U5 snRNP protein in 3' splice site selection. Genes & Develop. 9: 855-868. [PubMed citation]


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    1990 - 1994

  50. Madhani, H. and Guthrie, C. (1994) Dynamic RNA-RNA interactions in the spliceosome. Ann. Rev. Genet. 28: 1-26. [PubMed citation]


  51. Strauss, E. and Guthrie, C. (1994) PRP28, a "DEAD-box" protein, is required for the first step of mRNA splicing in vitro. Nucl. Acids Res. 22: 33187-3193. [PubMed citation]


  52. Madhani, H. and Guthrie, C. (1994) Randomization-selection analysis of snRNAs in vivo: Evidence for a tertiary interaction in the spliceosome. Genes & Devel. 8: 1071-1086. [PubMed citation]


  53. Madhani, H. and Guthrie, C. (1994) Genetic interactions betwen the RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase. Genetics, 137: 677-687. [PubMed citation]


  54. Frank, D., Roiha, H. and Guthrie, C. (1994) Architecture of the U5 snRNA. Mol. Cell. Biol. 14: 2180-2190. [PubMed citation]


  55. Lesser, C. and Guthrie, C. (1993) Mutations in U6 snRNA that alter splice site specificity: Implications for the active site. Science 262: 1982-1988. [PubMed citation]


  56. Burgess, S.M. and Guthrie, C. (1993) Beat the clock: Paradigms for NTPases in the maintenance of biological fidelity. Trends Biochem. Sci. 18: 381-384. [PubMed citation]


  57. Burgess, S.M and Guthrie, C. (1993) A mechanism for enhancing mRNA splicing fidelity by ATP hydrolysis: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates. Cell 73: 1377-1391. [PubMed citation]


  58. Lesser, C. F. and Guthrie C. (1993) Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1 . Genetics 133:851-863. [PubMed citation]


  59. Madhani, H. and Guthrie, C. (1992) A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell 71: 803-817. [PubMed citation]


  60. Schwer, B. and Guthrie, C. (1992) A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis. EMBO J.11: 5033-5039. [PubMed citation]


  61. Frank, D. and Guthrie, C. (1992) An essential splicing factor, SLU7, mediates 3’ splice-site choice in yeast. Genes & Devel. 6.: 2112-2124. [PubMed citation]


  62. Frank, D., Patterson, B. and Guthrie, C. (1992) Synthetic lethal mutations identify interactions between U5 snRNA and four proteins required for the second step of splicing. Mol. Cell. Biol. 21: 5197-5205. [PubMed citation]


  63. Schwer, B. and Guthrie, C. (1992) A dominant negative mutation in a splicesomal ATPase affects ATP hydrolysis, but not binding to the spliceosome. Mol. Cell. Biol. 12: 3540-3547. [PubMed citation]


  64. Bordonné, R. and Guthrie, C. (1992) Human and human-yeast chimeric U6 snRNA genes identify structural elements required for expression in yeast. Nucl. Acids Res. 20: 479-485. [PubMed citation]


  65. Siliciano, P.G., Kivens, W.J. and Guthrie, C. (1991) More than half of yeast U1 snRNA is dispensable for growth. Nucl. Acids Res. 19: 6367-6372. [PubMed citation]


  66. Guthrie, C. (1991) mRNA splicing in yeast: Clues to why the spliceosome is a ribonucleoprotein. Science 253: 157-163 . [PubMed citation]


  67. Shannon, K. and Guthrie, C. (1991) Suppressors of a U4 mutation define a novel U6 snRNP protein with RNA binding motifs. Genes & Develop. 5: 773-785. [PubMed citation]


  68. Strauss, E. and Guthrie, C. (1991) A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes & Develop. 5: 629-641. [PubMed citation]


  69. Schwer, B. and Guthrie, C. (1991) PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349: 487-493. [PubMed citation]


  70. Patterson, B. and Guthrie, C. (1991) A U-rich tract enhances usage of an alternative 3' splice site in yeast. Cell 64:181-187. [PubMed citation]


  71. Madhani, H., Bordonné, R. and Guthrie, C. (1990) Multiple roles for U6 snRNA in the splicing pathway. Genes & Develop. 4: 2264-2277. [PubMed citation]


  72. Jones, M.H. and Guthrie, C. (1990) Unexpected flexibility in an evolutionarily conserved protein:RNA interaction: Genetic analysis of the Sm binding site. EMBO J. 9: 2555-2561. [PubMed citation]


  73. Brow, D. and Guthrie, C. (1990) Transcription of the yeast U6 snRNA gene requires a pol III promoter element in a novel position. Genes & Develop. 4: 1345-1356. [PubMed citation]


  74. Bordonné, R., Banroques, J., Abelson, J. and Guthrie, C. (1990) Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions and pre-mRNA splicing in vivo. Genes & Develop. 4: 1185-1196. [PubMed citation]


  75. Shuster, E. and Guthrie, C. (1990) Human U2 snRNA can function in pre-mRNA splicing in yeast. Nature 345: 270-273. [PubMed citation]


  76. Burgess, S., Couto, J.R. and Guthrie, C. (1990) A putative ATP-binding protein influences the fidelity of branchpoint recognition in yeast splicing. Cell, 60: 705-717. [PubMed citation]


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    1985 - 1989

  77. Guthrie, C. (1989) Catalytic RNA and RNA splicing. Amer. Zoologist, 29: 557-567.

  78. Roiha, H., Shuster, E., Brow, D., and Guthrie, C.(1989) snRNAs from budding yeasts: Phylogenetic comparisons reveal extensive size variation. Gene, 82: 137-144. [PubMed citation]


  79. Brow, D.A. and Guthrie, C. (1989) Splicing a spliceosomal RNA. Nature, 337: 14-15. [PubMed citation]


  80. Siliciano, P. and Guthrie C. (1988) 5' splice site selection in yeast: genetic alterations in base pairing with U1 reveal additional requirements. Genes and Develop., 2: 1258-1267. [PubMed citation]


  81. Shuster, E. and Guthrie, C. (1988) Two conserved domains of yeast U2 snRNA are separated by 945 non-essential nucleotides. Cell, 55: 41-48. [PubMed citation]


  82. Guthrie, C. and Patterson, B. (1988) Spliceosomal snRNAs. Ann. Rev. Genetics, 22: 387-419. [PubMed citation]


  83. Brow, D. and Guthrie, C. (1988) Spliceosomal RNA U6 is remarkably conserved from yeast to mammals. Nature, 334: 213-218. [PubMed citation]


  84. Parker, R., Simmons, T., Shuster, E., Siliciano, P. and Guthrie, C. (1988) Genetic analysis of snRNAs in yeast: Viable sextuple mutant. Mol. Cell. Biol., 8: 3150-3159. [PubMed citation]


  85. Guthrie, C. (1987) Genetic analysis of yeast snRNAs. In: Structure and Function of Major and Minor snRNPs (ed. M. Birnstiel), Springer-Verlag, New York, pp. 196-211.

  86. Siliciano, P., Jones, M.H. and Guthrie, C. (1987) S. cerevisiae has a U1-like snRNA with unexpected properties. Science 237:1484-1487. [PubMed citation]


  87. Siliciano, P., Brow, D., Roiha, H. and Guthrie, C. (1987) An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA. Cell 50: 585-592. [PubMed citation]


  88. Couto, J., Tamm, J., Parker, R. and Guthrie, C. (1987) A trans-acting suppressor restores splicing of a yeast intron with a branchpoint mutation. Genes & Develop. 1: 445-455. [PubMed citation]


  89. Patterson, B. and Guthrie, C. (1987). An essential yeast snRNA with a U5-like domain is required for splicing. Cell 49: 613-624. [PubMed citation]


  90. Parker, R., Siliciano, P., and Guthrie, C. (1987) Recognition of the TACTAAC box during mRNA splicing in yeast involves base-pairing with the U2-like snRNA. Cell, 49: 229-239. [PubMed citation]


  91. Riedel, N., Wolin, S., and Guthrie, C. (1987) A subset of yeast snRNAs contain functional binding sites for the highly conserved Sm antigen. Science, 235: 328-331. [PubMed citation]


  92. Guthrie, C. (1986) Finding functions for small nuclear RNAs in yeast. Trends Biochem. Sci. 11: 430-434.

  93. Riedel, N., Wise, J.A., Swerdlow, H., Mak, A. and Guthrie, C. (1986) Small nuclear RNAs from Saccharomyces cerevisiae: Unexpected diversity in abundance, size, and molecular complexity. Proc. Natl. Acad. Sci. 83: 8097-8101. [PubMed citation]


  94. Vijayraghavan, U., Parker, R., Tamm, J., Cellini, A., Rossi, J., Abelson, J. and Guthrie, C. (1986) Mutations in conserved signals affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome. EMBO J. 5: 1683-1695. [PubMed citation]


  95. Cellini, A., Parker, R., McMahon, J., Guthrie, C. and Rossi, J. (1986) Activation of a cryptic TACTAAC box in the yeast actin intron. Mol. Cell. Biol., 6: 1571-1578. [PubMed citation]


  96. Guthrie, C., Riedel, N., Parker, R., Swerdlow, H., and Patterson, B. (1985) Genetic analyses of snRNAs and RNA processing in yeast. Yeast Cell Biology, UCLA Symposia on Molecular Biology, New Series, 33: 301-321.

  97. Tollervey, D. and Guthrie, C. (1985) Deletion of a yeast small nuclear RNA gene impairs growth. EMBO J. 4: 3873-3878. [PubMed citation]


  98. Parker, R. and Guthrie, C. (1985) A point mutation in the conserved hexanucleotide at the 5' splice junction of the yeast actin intron uncouples recognition, cleavage, and ligation. Cell 41: 107-118. [PubMed citation]


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