Herskowitz Lab Protocol
From : Megan Grether


Simple DAPI Staining Protocol

  1. sonicate
  2. spin cells down
  3. wash 1X PBS
  4. fix with 70% EtOH for 20minutes room temp.
  5. wash 1X PBS
  6. add DAPI (1mg/ml) 1:1000 dilution
  7. leave 12 minutes at room temp.
  8. wash 1X PBS

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