Herskowitz Lab Protocol
From : Sylvia Sanders
Ref: Methods in Enz. 194:94


Liquid Sporulation

  1. Grow cells overnight in YEPD
  2. Remove 200ml; add 5ml water
  3. Spin 5'
  4. r/s pellet in 2.5ml SPM
  5. incubate at 25°C with good aeration for 2-4 days
  6. Take 1ml, r/s in 100ml zymolase -- dissect

SPM

3g/L KOAc
0.2g/L Raffinose

 


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