PROTOCOLS

YEAST GENETICS

• Theory & Practice
  • A Reading List for starting a yeast genetics project : the necessary and the eclectic.
• Useful Templates for Yeast Genetics
  • Patch Pattern for 10 tetrads (a useful template for patching
    yeast onto plates.)
  • Scoring Sheet for 10 tetrads (a useful page for keeping records
    of yeast dissections.)
• Yeast Genetic Protocols
  • Yeast Transformation
  • Sleazy Plate Transformation of Yeast
  • PCR to check genotype
  • Bgal filter assay
  • Bgal plate overlay assay
  • Bgal liquid assay
  • Liquid Sporulation
  • EMS Mutagenesis
  • Generating a Temperature Sensitive Allele
  • Yale Genome Analysis Center
• Sequences
  • pNKY51 (hisG.URA3.hisG cassette on a BamHI- BglII fragment
    from the lab of Nancy Kleckner)
  • YEp24-CYC1-LacZ (classic vector for assaying upstrea m regions
    on a heterologous promoter from Lenny Guarente's lab)

YEAST CELL BIOLOGY

• Immunofluorescence for Epitope Tagged Proteins
• Dapi Staining
• FACS Analysis

YEAST BIOCHEMISTRY

• Extracts for Yeast Westerns (M.P.)
• Silver Staining Protocol (Diamine Staining)

Protocols from Other Labs