Guthrie & Fink [Volume 194 of Methods of Enzymology] provides a detailed and comprehensive description of protocols and techniques. It covers methods from rescuing mutant alleles to preparing yeast nuclei.

For well written reviews of multiple aspects of yeast biology, consult Cold Spring Harbor Press. Selected books and monographs can provide a foundation of knowledge from cell cycle to metabolism.

Crash Courses

We also highly recommend for the very serious and those who can afford it the Cold Spring Harbor Yeast Course (another link to this course). Another way to be immersed in this study is to attend the Yeast Genetics Meeting or the Cold Spring Harbor Yeast Cell Biology Meeting.

An Eclectic List from Ira

Ira chose papers that should not require a vast amount of background material so that you can more easily see the questions that have been posed and the genetic strategies used to address the questions.

• Different strategies for screening for mutants

Chant J; Herskowitz I.Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway.Cell, 1991 Jun 28, 65(7):1203-12. [A brute force microscopic screen.]

Wilson KL; Herskowitz I.STE16, a new gene required for pheromone production by a cells of Sac charomyces cerevisiae.Genetics, 1987 Mar, 115(3):441-9.[A sophoisticated screening method used to simplify later analysis.]

• Ordering gene function within a pathway

Toh-e A; Inouye S; Oshima Y.Structure and function of the PHO82-pho4 locus controlling the synthesis of repressible acid phosphatase of Saccharomyces cerevisiae. Journal of Bacteriology, 1981 Jan, 145(1):221-32.

Stevenson BJ; Rhodes N; Errede B; Sprague GF Jr.Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein.Genes and Development, 1992 Jul, 6(7):1293-304.[beautiful epistasis analysis]

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• Additional strategies for identifying genes

-General strategies

Li JJ; Herskowitz I.Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system Science, 1993 Dec 17, 262(5141):1870-4.

Burns N; Grimwade B; Ross-Macdonald PB; Choi EY; Finberg K; R oeder GS; Snyder M.Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae.Genes and Development, 1994 May 1, 8(9):1087-105.

Use of suppressorsSprague GF Jr; Herskowitz I.Control of yeast cell type by the mating type locus. I. Identif ication and control of expression of the a-specific gene BAR1. Journal of Molecular Biology, 1981 Dec 5, 153(2):305-21.

-Use of high copy plasmids

Kurjan J; Herskowitz I.Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.Cell, 1982 Oct, 30(3):933-43.

Ogas J; Andrews BJ; Herskowitz I. Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription. Cell, 1991 Sep 6, 66(5):1015-26.

• An example : Control of initiation of sporulation

Here, Ira listed a series of papers so that you can see how the work has progressed from genetic analysis to biochemical tests.

Rine J; Sprague GF Jr; Herskowitz I. rme1 Mutation of Saccharomyces cerevisiae: map position and bypass of mating type locus control of sporulation. Mol Cell Biol, 1981 Oct, 1(10):958-60.
[A genetic test of two different hypotheses for RME1]

Mitchell AP; Herskowitz I. Activation of meiosis and sporulation by repression of the RME1 product in yeast. Nature, 1986 Feb 27-Mar 5, 319(6056):738-42.[A biochemical test using the cloned RME1 gene.]

Covitz PA; Herskowitz I; Mitchell AP.The yeast RME1 gene encodes a putative zinc finger protein that is directly repressed by a1-alpha 2.Genes and Development, 1991 Nov, 5(11):19

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