Lab BBQ, July 2007
Our lab is interested in the engineering design principles that underly cellular morphogenesis. We want to understand how the three-dimensional geometry of cells can be encoded in a one-dimensional genome and developed into varying cellular architectures under genetic control. We are investigating this question at the level of individual organelles (centrioles, cilia, and mitochondria) as well as through studies of the overall architecture of cells. We are particularly interested in the control of organelle size and copy number, and in the role of biological self-replication processes. Our lab uses a combination of genomic, proteomic, imaging, and mathematical modeling approaches.
How Cells Count
- mechanism of centriole duplication
- regulation of centriole copy number
- how the centriole proteome assembles
- centriole function in cell division and organization
How Cells Measure
- organelle size control systems
- ciliary disease genes and length control
How Cells Tell Direction
- left/right symmetry breaking by ciliary movement
- how centrioles find position within cells
Marshall WF. 2007.
Stability and robustness of an organelle number control system: modeling and measuring homeostatic regulation of centriole abundance.
Biophysical Journal 93,1818-1833.
Feldman JL, Geimer S, Marshall WF. 2007.
The mother centriole plays an instructive role in defining cell geometry.
PLoS Biology 5,e149.
Keller LC, Romijn EP, Zamora I, Yates JR, Marshall WF. 2005. Proteomic analysis of isolated Chlamydomonas centrioles reveals orthologs of ciliary disease genes.
Curr. Biol. 15,1090-8.
Nonaka S, Yoshiba S, Watanabe D, Ikeuchi S, Goto T, Marshall WF, Hamada H. 2005.
De novo formation of
left-right asymmetry by posterior tilt of nodal cilia.
PloS Biology 3,e268.
Zamora I, Marshall WF. 2005. A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii.
BMC Biol. 3,15.
Stolc V, Samanta MP, Tonprasit W, Marshall WF. 2005. Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes.
PNAS 102,3703-7.
Marshall WF. 2004. Human cilia proteome contains homolog of zebrafish polycystic kidney disease gene qilin.
Curr. Biol. 14,R913-4.
Marshall WF, Qin H, Rodrigo Brenni M, Rosenbaum JL. 2005. Flagellar length control system: testing a simple model based on intraflagellar transport and turnover.
Mol. Biol. Cell 16,270-8.
Zamora I, Feldman JL, Marshall WF. 2004. PCR-based assay for mating type and diploidy in Chlamydomonas.
Biotechniques 37,534-6.
Marshall WF. 2004. Cellular length control systems.
Ann. Rev. Cell Dev. Biol. 20,677-93.
Juliette Azimzadeh, Postdoc
Benjamin Engel, Grad Student
Jessica Feldman, Grad Student
Hiroaki Ishikawa, Postdoc
Elisa Kannegaard, Grad Student
Lani Keller, Grad Student
Will Ludington, Grad Student
Judy Piccini, Admin Assistant
Susanne Rafelski, Postdoc
Kim Wemmer, Grad Student
Ivan Zamora, SRA
Shigenori Nonaka, former Postdoc, currently assistant Professor, National Institute for Basic Biology, Okazaki Japan
Christopher Baker, former SRA, currently grad student at UCSF Tetrad Program
Roshan Karki, former SRA, currently grad student at Yale MCDB Program
Afsaneh Zolfaghari, former SRA, currently grad student at Harvard School of Public Health
GH-N376 Genentech Hall
Department of Biochemistry & Biophysics
UCSF Mission Bay
600 16th St.
San Francisco, CA 94143-2200
(415) 514-4323
UCSF Program in Quantitative Biology
Building the Cell - workshop on the engineering design principles of cellular morphogenesis